FLOCCULATION METHOD

摘要

The present invention relates to a method for harvesting recombinant proteins from mammalian cell culture fluid. The method makes use of cationic polymers, non-ionic polymers and non-ionic surfactants.

主权项

1. A mammalian cell culture harvest method comprising culturing mammalian cells expressing a recombinant protein in a cell culture medium for a predetermined time or until a desired cell density and/or packed cell volume is achieved,
adding a cationic polymer and a non-ionic polymer to the cell culture medium initiating flocculation, mixing the cell culture medium during flocculation, allowing the flocculent to settle for a primary settle, and recovering the primary clarified supernatant.