METHOD FOR PREPARING CELLULARIZED CONSTRUCTS BASED ON HEAT-SENSITIVE HYDRO-GELS
摘要
A method is described for preparing cellularized constructs through quick prototyping comprising the following steps: (A) preparing a polymeric solution comprising one or more natural and/or synthetic polymers in a culture medium at a temperature included between 1°C and 20°C; (B) dispersing cells inside the polymeric solution; (C) gelling the polymeric dispersion containing said cells by increasing the temperature to a value included between 36°C and 38 °C in order to obtain a cellularized hydro-gel; (D) extruding the cellularized hydro-gel through a suitable nozzle on a thermostated support at a temperature included between 36°C and 38 °C.
申请公布号
WO2016194011(A1)
申请公布日期
2016.12.08
申请号
WO2016IT00142
申请日期
2016.06.01
申请人
POLITECNICO DI TORINO;UNIVERSITA' CAMPUS BIO-MEDICO DI ROMA
发明人
CHIONO, Valeria;SARTORI, Susanna;BOFFITO, Monica;GIOFFREDI, Emilia;TROMBETTA, Marcella;MOZETIC, Pamela;RAINER, Alberto;GIANNITELLI, Sara Maria