METHOD FOR PROTEIN PURIFICATION COMPRISING HEAT INCUBATION IN ACETIC ACIDIC SOLUTION

摘要

The present invention relates to a novel process for purifying a recombinant protein including one or a few procedural steps only. The process according to the invention is characterised by that it combines the step of lysis of the host cell, with the purification of the protein of interest, allowing for a rapid and much more efficient process of purification than previously available within the field of the art. The conditions used during the purification process are those of a high temperature and a low pH, allowing for thermostable and acid-resistant recombinant proteins to be isolated from a suspension. The invention also relates to purifying recombinant proteins which are fusion proteins, wherein one part of the protein may be selected from an enamelmatrix protein, such as amelogenin.