FILTER SYSTEMS FOR SEPARATING MICROCARRIERS FROM CELL CULTURE SOLUTIONS

摘要

A filter assembly for separating microcarriers from a fluid medium includes a collapsible container bounding a sterile compartment adapted to hold a fluid. An inlet port is attached to the container through which fluid flows into the compartment. An outlet port is attached to the container through which fluid flows out of the compartment. A filter is disposed within the compartment, the filter dividing the compartment into an inlet chamber that is fluidly coupled with the inlet port and an outlet chamber that is fluidly coupled with the outlet port, the filter allowing a medium to pass therethrough but preventing microcarriers disposed in the medium from passing therethrough.

主权项

1. A filter assembly for separating microcarriers from a medium in which the microcarriers have been added to aid in cell culture development, the filter assembly comprising:
a container bounding a sterile compartment adapted to hold a fluid; a filter port attached to the container, the filter port having a fluid passageway extending therethrough such that a solution having microcarriers therein can pass therethrough; a filter disposed within the sterile compartment of the container, the filter comprising:
a bag or sock bounding a chamber and being at least partially formed from a porous material so that a solution that includes cells can pass therethrough while microcarriers are prevented from passing therethrough; andan inlet port coupled to the bag or sock and communicating with the chamber; and a tube extending between the filter port and the inlet port.