Microscale bioprocessing system and method for protein manufacturing

摘要

A bioprocessing system for protein manufacturing is provided that is compact, integrated and suited for on-demand production and delivery of therapeutic proteins to patients. The system can also be used for efficient on-demand production of any type of protein.

主权项

1. A bioprocessing system, comprising:
a reactor adapted for cell-free protein expression that produces a protein utilizing cell-free protein expression; at least one sensor for monitoring reaction parameters in the reactor; and a purification module for receiving the protein from the reactor and for purifying the protein from reagents, wherein the purification module comprises,
a chromatography component for receiving the protein from the production module and for outputting purified protein, anda diafiltration component comprising a product section for receiving the purified protein from the chromatography component and a buffer section for receiving a buffer solution, wherein the diafiltration component outputs further purified protein, and wherein the diafiltration component further comprises,
a first substrate,a second substrate, anda diafiltration membrane positioned between the first and second substrates.