Fused NHase with improved specific activity and stability

摘要

The present invention provides a fused NHase with improved specific activity and stability, which relates to the field of genetic engineering. This invention provides a method of overexpressing a fused NHase in E. coli and producing a mutant NHase with improved stability and product tolerance. The invention provides a simple, efficient and safe method of making mutant NHase, and can produce a large amount of soluble NHases in a short period. The present invention makes a contribution to large-scale industrial production and further theoretical study of NHases.

主权项

1. A mutant Nitrile hydratase (NHase) with improved specific activity and stability, wherein said mutant NHase comprises a β-subunit fused together with an α-subunit, and a regulatory subunit that coexpresses with the fused α-βsubunit.