Diagnosis kit and chip for bladder cancer using bladder cancer specific methylation marker gene

摘要

The present invention relates to a kit and nucleic acid chip for diagnosing bladder cancer using a bladder cancer-specific marker gene. More particularly, the invention relates to a kit and nucleic acid chip for diagnosing bladder cancer, which can detect the promoter methylation of a bladder cancer-specific gene, the promoter or exon region of which is methylated specifically in transformed cells of bladder cancer. The use of the diagnostic kit or nucleic acid chip of the invention enables diagnosis of bladder cancer at an early stage of transformation, thus enabling early diagnosis of bladder cancer, and can diagnose bladder cancer in a more accurate and rapid manner compared to a conventional method.

主权项

1. A method for detecting CpG methylation of SIM2-single-minded homolog 2(Drosophila) gene - for bladder carcinoma or bladder cell proliferative disorder diagnosis in a human subject, the method comprising determining hypermethylation of the CpG of the SIM2 gene in genomic DNA isolated from a clinical sample by contacting the sample with primer(s) comprising a DNA sequence selected from the group consisting of SEQ ID NO: 19, 20, 41 and 42 to amplify a methylated CpG of the SIM2 gene, wherein a bladder carcinoma or bladder cell proliferative disorder is detected in the human subject based on increased CpG methylation of the SIM2 gene relative to that of a control.