Methods for producing a triglyceride composition from algae

摘要

Methods are disclosed for cultivation of oleaginous microorganisms on feedstocks including depolymerized cellulosic material such as corn stover, Miscanthus, forage sorghum, sugar beet pulp and sugar cane bagasse. Also disclosed are methods of manufacturing novel triglyceride oil compositions using cellulosic feedstocks, including oil compositions comprising fatty acids such as those having chain lengths of 8, 10, 12, and 14 carbon atoms.

主权项

1. A method of producing a lipid composition comprising:
(a) providing a culture medium with a population of Prototheca cells, the cells comprising an exogenous gene operably linked to a promoter sequence selected from the group consisting of SEQ ID NO: 96, SEQ ID NO: 97, and SEQ ID NO: 98, wherein the exogenous gene is heterologous to the promoter sequence; (b) adding depolymerized cellulosic material to the culture medium, wherein the added depolymerized cellulosic material has a glucose concentration of at least 600 g/L and a salt concentration of less than 300 mM; (c) cultivating the population of cells heterotrophically in the dark thereby accumulating lipid in the cells; and (d) isolating lipid from the cells to provide the lipid composition.