| 摘要 |
A device capable of obtaining a sample from an animal or human and processing the sample to detect a molecule of interest. The device comprises a two-sided needle, a sample collection tube that interconnects with a second tube containing processing agents via a compatible threaded coupling present on each tube. A compatible detection tube is provided. The present invention also relates to methods detecting defective prions in a sample obtained from an animal or a human by obtaining a sample, contacting the sample with proteinase K/cell lysing solution to form a mixture that will digest extraneous proteins in the sample but leave defective prions that may be in the sample unaffected, adding a monoclonal antibody specific for defective prions to the mixture, and detecting defective prions. The present invention also relates to method of detecting BSE in an animal prior to destruction of the animal comprising detecting defective prions as described above where the presence of defective prion proteins indicates the presence of BSE.
|
