发明名称 |
СПОСОБ ПОЛУЧЕНИЯ ИНТЕРЛЕЙКИНА-8 ИЗ НЕЙТРОФИЛОВ КРОВИ ДОНОРОВ |
摘要 |
FIELD: medicine, immunology, cytokines. ^ SUBSTANCE: method for preparing interleukin-8 (L-8) from donor blood neutrophiles involves isolating neutrophiles from peripheral blood in double gradient ficol-verografin, bringing about the neutrophile content in cell suspension to the concentration 5 x 106 cells/ml, activation at temperature 37°C for 1 h followed by centrifugation and separation of supernatant containing the end product. For activation the cell suspension of neutrophiles in the concentration 5 x 106/ml of Henx's solution is poured in plastic Petri dishes. Invention provides the development of a simple and available method providing the high level of interleukin-8 (IL-8) from neutrophiles of donor blood not requiring using expensive preparations. ^ EFFECT: improved preparing method. ^ 1 tbl |
申请公布号 |
RU2005118285(A) |
申请公布日期 |
2006.12.20 |
申请号 |
RU20050118285 |
申请日期 |
2005.06.14 |
申请人 |
Северо-Осетинска государственна медицинска академи (RU) |
发明人 |
Треть кова Ирина Евгеньевна (RU) |
分类号 |
A61K38/20;A61K35/14;C07K14/55 |
主分类号 |
A61K38/20 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|