COMPOUNDS AND METHODS FOR PEG METABOLITE AND PEG BREAKDOWN PRODUCT ASSAYS

摘要

Disclosed herein are compounds and methods for identifying and quantifying a metabolite or breakdown product of PEG. A sample may be assayed for PEG metabolites or breakdown products using liquid chromatography combined with mass spectrometry. Derivatization of the PEG metabolites or breakdown products within the sample with pentaflurobenzoyl chloride in conjunction with negative chemical ionization mode liquid chromatography optimizes the assay.

主权项

1. A method for detecting and quantifying a compound, said method comprising
obtaining a sample, said sample containing one or more of ethylene glycol and diethylene glycol; combining said sample, an aqueous hydroxide containing salt, and pentaflurobenzoyl chloride dissolved in an organic solvent thus producing a biphasic liquid system, said biphasic liquid system comprising a pentafluorobenzoate ester derivative; separating a liquid phase supernatant from said biphasic liquid system; and analyzing said supernatant using liquid chromatography and mass spectrometry (LC/MS) to detect said pentafluorobenzoate ester derivative.