New methods to produce active tert

摘要

The present invention relates to a method to reproducibly produce large amounts of soluble and active hTERT by expressing this enzyme in yeast cells. High yield of recombinant active hTERT has been obtained by controlling specific parameters of the purification process. The hTERT protein thus obtained displays telomerase activity with in vitro transcribed hTR, opening the way to high throughput chemical screening of telomerase assembly inhibitors and therapeutic applications.