Device and method for production and analysis of prions

摘要

The invention provides a method for producing prion protein having an aggregated conformation by contacting native conformation prion protein with aggregated conformation prion protein in a liquid preparation and subjecting this to at least one cycle or to a number of cycles of application of shear-force for fragmenting aggregates of prion protein, wherein the shear-force applied is precisely controlled. In addition to this process for amplification of aggregated state prion protein from native conformation prion protein, the invention relates to the aggregated state prion protein obtained by the amplification process, which aggregated state prion protein has one conformation, which is e.g. identical within one batch and reproducible between batches, e.g. as detectable by proteinase resistance in a Western blot.

主权项

1. A process for amplification of aggregated conformation prion protein from native conformation prion protein, comprising:
contacting the native conformation prion protein with the aggregated conformation prion protein in a liquid to form a liquid composition; and subjecting the liquid composition to at least one cycle comprising the generation of shear force for fragmenting the aggregated conformation prion protein and a resting phase, wherein the shear force is applied to each volume element of the liquid composition and consists of a uniform shear-force intensity having a value within ±10% of a given shear-force value, wherein the shear-force is generated by rotation of a rotary element arranged within a spaced coaxial wall and passing each volume element between the rotary element and the wall while the rotation is controlled to within ±10% of one rate.