CULTURE CONTAINER, METHOD FOR CULTURING LYMPHOCYTES, CULTURE-CONTAINER PRODUCTION METHOD, AND SOLID-PHASING APPARATUS

摘要

A culture container for culturing lymphocytes, wherein the culture container comprises a gas permeable film, antibodies are immobilized on only one of the container inner surfaces facing each other, thereby to provide a immobilized surface and a non-immobilized surface, and anti-CD3 antibodies are immobilized in the immobilized surface at a concentration of 10 to 300 ng/cm 2 . Lymphocytes and a culture liquid are sealed in this culture container, and the culture container is arranged such that the immobilized surface in the culture container becomes a bottom surface, thereby to activate lymphocytes and then the culture container is inverted upside down and arranged such that the non-immobilized surface in the culture container becomes a bottom surface, thereby to proliferate lymphocytes. Such a culture container in which proteins are immobilized can be produced by injecting into a culture container liquid droplets containing proteins dissolved therein and then the liquid droplets are moved on part or the whole on the inner surface of the culture container.