| 摘要 |
<p>The present invention discloses methodologies for negative selection of specific phagocytic cells from a heterogeneous population of cells derived from bone marrow and/or peripheral blood utilizing a ferrous-based, preferably carbonyl iron, magnetic selection techniques. Carbonyl iron (CI) selection allows for efficient removal, via the use of a high-energy magnet (e.g., samarium cobalt), of cells such as macrophages, monocytes, and neutrophils without any associated decrease in the number of hematopoietic CD34+ stem or myeloid progenitor cells. CI selection results in the removal of > 40 % of the total number of cells, and a concomitant removal of > 85 % of monocytes in bone marrow samples. Similarly, in peripheral blood samples, CI selection results in the removal of 68 % of the total number of cells, with a concomitant 6-fold enrichment of CD34+. Moreover, when CI selection is combined with Ficoll-Hypaque density gradient centrifugation, a depletion of > 85 % of total cell number and a concomitant 9-fold enrichment of CD34+ cells is found in peripheral blood samples. Following carbonyl iron selection, the enriched cell population may then be subjected to further positive selection of hematopoietic CD34+ stem/progenitor cells and/or negative selection of contaminating, malignant cells utilizing magnetic immunobead methodology.</p> |
