METHOD FOR THE CONTINUOUS PRODUCTION OF CELLULASES BY A FILAMENTOUS FUNGUS USING A CARBON SUBSTRATE OBTAINED FROM AN ACID PRETREATMENT

摘要

The present invention relates to a method for producing cellulases and hemicellulases by a strain belonging to a filamentous fungus in an agitated and aerated bioreactor including at least two phases i.e. a phase a) for growing said strain in the presence of at least one carbon growth substrate in a closed reactor said growth phase being carried out with a concentration of carbon growth substrate of between 10 and 90 g/L a phase b) for the continuous production of cellulases in which at least one carbon inducing substrate is fed at an at least constant feed rate for a period at least greater than 200 hours said carbon substrate being at least an aqueous hemicellulose hydrolysate solution derived from an acid pretreatment of a lignocellulose substrate said aqueous hemicellulose hydrolysate solution not being presterilized the pH thereof not being corrected said pH of the aqueous solution being between 0.5 and 3 wherein the weight of the reaction volume is kept constant by drawing off a fraction of said reaction volume said phase b) being carried out at a dilution rate of between 0.001 and 0.02 h 1.