ALDOSE REDUCTASE INHIBITOR BY YUA25-005

摘要

PURPOSE: Aldose reductase inhibitor by YUA25-005 is useful to treat complication generated from glycosuria. CONSTITUTION: Aldose reductase inhibitor by YUA25-005 is screening enzyme inhibition activity with supernatant getting by contrifuging after shaking culture specific environmental microorganisms isolated from soil in 250ml conical flask, cultivating alkalipholic strain Bacillus sp. YUA17 with high inhibition activity in said screening in 200 liter fermenter for 48 hours and obtaining broth 200 liter, removing impurity by ion exchange resin and adsorption resin of supernatant, obtaining aldose reductase inhibitor(YUA005) by refining ethylacetate extracts using silicagel column chromatography, spectroscopic analyzing YUA005 like allochemical analysis and nuclear magnetic resonance spectrum, mass spectrum.