MONOVALENT ANTIGEN BINDING CONSTRUCTS TARGETING EGFR AND/OR HER2 AND USES THEREOF

摘要

Provided herein are monovalent antigen-binding constructs targeting EGFR and/or HER2. The monovalent antigen-binding constructs can include at least one antigen-binding polypeptide comprising a heavy chain variable domain, wherein the antigen-bind polypeptide specifically binds EGFR and/or HER2; and a heterodimeric Fc domain, the Fc domain comprising at least two CH3 domains, wherein the Fc domain is coupled, with or without a linker, to the antigen-binding polypeptide. Also provided are methods of making the constructs and methods of using the constructs.

主权项

1. A method of treating a subject having an epidermal growth factor receptor (EGFR)-expressing tumor, comprising: contacting the tumor with an effective amount of an isolated monovalent EGFR-binding construct comprising at least one antigen-binding polypeptide comprising a heavy chain variable domain coupled, with or without a linker, to a heterodimeric Fc, wherein the antigen-binding polypeptide binds or specifically binds to EGFR, and wherein the construct binds to EGFR with a greater Bmax as compared to the corresponding isolated monospecific bivalent antigen-binding construct that binds or specifically binds EGFR.