AGENTS, METHODS AND KITS FOR TREATING AND DETECTING DIABETIC NEPHROPATHY AND PROLIFERATIVE DISEASES AND METHODS FOR INHIBITING EXPRESSION OF TYPE IV COLLAGEN

摘要

A method of detecting proliferative diseases causing sclerosis, comprising measuring the expression of at least one substance selected from the group consisting of STAT3, phosphorylated STAT3, Smad1, phosphorylated Smad1, activin receptor-like kinase 1, activin receptor-like kinase 3 and bone morphogenetic proteins in a biological sample. A kit therefor. A prophylactic and/or therapeutic agent for proliferative diseases causing sclerosis, comprising as an active ingredient a substance having an inhibitory effect on the expression of at least one substance selected from the group consisting of STAT3, phosphorylated STAT3, Smad1 and phosphorylated Smad1. A method of identifying substances effective in preventing and/or treating proliferative diseases causing sclerosis, comprising judging whether or not a test substance inhibits the expression of at least one substance selected from the group consisting of STAT3, phosphorylated STAT3, Smad1 and phosphorylated Smad1. A kit therefor.

主权项

1. A method of detecting and treating diabetic nephropathy, which method comprises:
contacting a biological sample from a subject with a pair of primers that amplify Smad1 and/or a substance having Smad1-activating effect; detecting the presence or absence of expression of Smad1 and/or a substance having Smad1-activating effect in the biological sample from the subject; identifying the subject as having diabetic nephropathy if the presence of the expression of Smad1 and/or the substance having Smad1-activating effect is detected; administering to the subject a drug comprising a substance having an inhibitory effect on the expression of Smad1, wherein the substance having Smad1-activating effect is selected from the group consisting of activin receptor-like kinase 1 (ALK1), activin receptor-like kinase 3 (ALK3) and bone morphogenetic protein BMP2.