PREPARATIVE CHROMATOGRAPHY COLUMN AND METHODS

摘要

A chromatography column that captures components in a process liquid in a free flow state and allows elution in steps is described.

主权项

1. A method of chromatographic separation of a desired component comprising:
a. Providing a disposable preparative chromatography apparatus comprising:
i. a flexible disposable bag with an inner volume;ii. a septum disposed within the flexible disposable bag to separate the flexible disposable bag into a flexible upper chamber and a flexible lower chamber, the septum including a porous surface that allows passage of liquid but not chromatography media from the upper chamber to the lower chamber;iii. a chromatography media disposed in the upper chamber;iv. at least one inlet to introduce liquid into the flexible upper chamber of the bag containing the chromatography media;v. at least one outlet to remove the liquid from the flexible lower chamber of the bag after passage through the septum; andvi. at least one mixing mechanism to mix the contents of the bag; b. Introducing a quantity of liquid containing a component to be separated into the upper chamber of the flexible bag through the inlet of (iv) above; c. Mixing the contents of the flexible bag to allow the component to bind to the chromatography media; d. Opening the drain in the lower chamber and draining out the liquid that has passed through the septum; e. Closing the drain; f. Introducing into the upper chamber a solution capable of disrupting the binding between the component and the chromatography media; g. Mixing the contents of the column until the component has been sufficiently released from the chromatography media; h. Opening the drain in the lower chamber; i. Collecting the contents of the flexible bag as a concentrated solution of the component.