ARTIFICIAL FEEDING METHOD AT LOW ALTITUDE FOR HOST INSECT GHOST MOTH OF OPHIOCORDYCEPS SINENSIS

摘要

An artificial feeding method at low altitude for a host insect ghost moth of Ophiocordyceps sinensis. This method mainly includes: selecting an appropriate disinfectant for performing surface disinfection on ghost moth eggs, and then placing the disinfected eggs in sterile humus; feeding ghost moth larvae with carrots, disinfecting and detecting the fed carrots, and culturing the larvae till male and female pupae are obtained; identifying male and female pupae of the ghost moths, placing them at different culture temperatures, wherein the culture temperature for the male pupae is 2-6° C. lower than that of the female pupae, resulting synchronously emerged male and female adults; placing the emerged male and female adults in an adult cage with simulated plants for mating and staying of the adults, maintaining a humidity over 80%, and employing 50-100 Lux shimmer for facilitating activity and mating of the adults, thus improving fertilization opportunity and hatchability of eggs when the adults after mating lay eggs.

主权项

1. An artificial feeding method at low altitude for a host insect ghost moth of Ophiocordyceps sinensis, comprising the following steps:
(a). managing an egg stage by performing surface disinfection a first egg laid by ghost moth adults to kill bacteria and control fungi to form a disinfected egg and, then placing the disinfected egg in sterile humus: (b). managing a larva stage by feeding ghost moth larvae with carrots, disinfecting and detecting the carrots to ensure that the carrots are harmless to ghost moth larvae, thus obtaining male pupae and female pupae; (c). managing a pupa stage by identifying the male pupae and the female pupae of ghost moths, placing the male pupae and the female pupae at different culture temperatures, wherein the culture temperature of the male pupae is 2-6° C. lower than that of the female pupae, such that development of the male pupae becomes slow and is synchronous with that of the female pupae; and (d). managing an adult stage by placing male adults and female adults that have emerged from the pupa stage (c) in an adult cage in which simulated plants are placed for mating and staying of the male adults and the female adults, maintaining a humidity over 80%, employing 50-100 Lux shimmer for facilitating activity and mating of the male adults and the female adults, and collecting a second egg after the female adults lay the second egg.