SRM/MRM Assay for the Fibroblast Growth Factor Receptor 2 (FGFR2) protein

摘要

Methods are provided for quantifying the fibroblast growth factor receptor 2 protein (FGFR2) directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM)/Multiple Reaction Monitoring (MRM) mass spectrometry. The biological sample may be selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells. A protein sample is prepared from the biological sample and the FGFR2 protein is quantitated in the sample using the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one fragment peptide derived from FGFR2.

主权项

1. A method for measuring the level of the fibroblast growth factor receptor 2 protein (FGFR2) in a biological sample, comprising detecting and/or quantifying the amount of one or more modified or unmodified FGFR2 fragment peptides in a protein digest prepared from said biological sample using mass spectrometry; and calculating the level of modified or unmodified FGFR2 protein in said sample; and
wherein said level is a relative level or an absolute level.