| 摘要 |
A nanoparticle probe is attached to a substrate to capture targets. The nanoparticle probe includes a specific binding agent that specifically binds to a target biomolecule. The biomolecule can be associated with a cell, for example expressed on the cell's surface, such that the cell is bound to the probe immobilized on the substrate. The nanoparticle probes can be applied to the substrate in a layer, for example in the form of a spot, and multiple spots can be applied to the substrate to form patterns or arrays of the spots on the substrate. The nanoparticle probe presents a binding surface on which oriented specific binding agents (such as antibodies or nucleic acids) can be attached. In particular examples the nanoparticle is spaced slightly from the substrate, for example by a linker, to provide a probe with improved contact with a liquid in which target biomolecules or cells are suspended. The probes can be applied to the substrate in identifiable locations, either by applying the nanoparticle probes to the substrate at a predetermined address or using a nanoparticle probe that emits a signal to identify its location. Particular examples of such probes are semiconductor nanocrystals such as quantum dots, which emit fluorescence of a particular color. The nanoparticle probes can sort biomolecules or cells of different types or subtypes, and maintain them in a substantially fixed location on the substrate where they can be studied for prolonged periods of time.
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