POLYPEPTIDES HAVING PULLULANASE ACTIVITY COMPRISING THE X25, X45 AND CBM41 DOMAINS

摘要

The present invention relates to a method of increasing pullulanase activity of a parent pullulanase comprising the steps: a) inserting an X25 domain within an X45 domain; and /or b) adding a CBM41 domain either adjacent to the N-terminal of an X45 domain, or if a CBM41 domain is already present, adding the additional CBM41 domain adjacent to the N-terminal of the existing CBM41 or adjacent to the N-terminal of the X45 domain.