Synthetic gene coding for influenza hemagglutinin

摘要

A process for the production of a synthetic gene for an influenza haemagglutinin which comprises: (a) subjecting isolated vRNA to the action of reverse transcriptase to produce a double-stranded RNA/DNA hybrid; (b) digesting the RNA component of the hybrid; (c) treating the residual DNA component of the hybrid to produce a double-stranded DNA molecule having one "hairpin" end; and (d) treating the "hairpin" structure with a single-strand specific nuclease to produce a bimolecular double-stranded copy of the vRNA.