| 摘要 |
A method of measuring lipid-bound sialic acid (LSA) which comprises, mixing a LSA separating agent comprising a polar solvent with a sample containing LSA and other sialic acid(s), separating the precipitate formed to obtain a supernatant, and measuring the sialic acid concentration of said supernatant by using an enzyme capable of acting on sialic acid in a lipid-bound state. When a sample is treated with the LSA separating agent comprising a polar solvent, various components in the sample such as protein-bound sialic acid and proteinous substances precipitate while the desired lipid-bound sialic acid remains in the supernatent. Accordingly, by measuring sialic acid concentration of the supernatent, LSA concentration of the sample is easily determined. The method of the invention is simple and superior in reproducibility. The method of the invention is suitable for treating a large number of samples, and it can easily by automated. This method is suitable as a screening test for cancer.
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