发明名称 |
METHODS FOR PRODUCING TAXANE-TYPE DITERPENES |
摘要 |
The present invention discloses a method for producing a taxane-type diterpene(s) by culturing cells of a taxane-type diterpene-producing plant, comprising one or both of the the following steps (a) and (b) to increase the ratio of those cell clusters suitable in size for diterpene production: (a) at least one operation for removing large cell clusters is conducted with a sieve and/or a filter during preculture of the cells to be supplied to main culture for the production of taxane-type diterpenes or at the time of cell transfer from one preculture to the subsequent preculture; (b) the cells are cultured under strong agitation conditions. The present invention also discloses a method for producing a taxane-type diterpene(s) by culturing cells of a taxane-type diterpene-producing plant, comprising preculturing the cells in a liquid medium under conditions of enriched oxygen supply, supplying the resultant cells to main culture for the production of a taxane-type diterpene(s) and recovering the taxane-type diterpene(s) from the resultant culture. According to the present invention, it is possible to improve the productivity of taxane-type diterpenes in the culture of cells of a taxane-type diterpene-producing plant.
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申请公布号 |
CA2241569(A1) |
申请公布日期 |
1998.12.25 |
申请号 |
CA19982241569 |
申请日期 |
1998.06.24 |
申请人 |
MITSUI CHEMICALS, INC. |
发明人 |
HARA, YASUHIRO;MATSUBARA, KOUICHI;YUKIMUNE, YUKIHITO |
分类号 |
C12N5/04;C12P7/02;(IPC1-7):C12P17/02 |
主分类号 |
C12N5/04 |
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