| 摘要 |
<p>A method of direct insertion of double-stranded RNA into a cloning vector consisting of double-stranded DNA, the method comprising: a) isolating the dsRNA from a source of interest; b) if required, decapping the dsRNA; and c) directly ligating the dsRna to the dsDNA of the appropriate cloning vector. This method allows dsRNA to be cloned directly, thus eliminating the traditional step of first making a complementary DNA copy of the RNA molecule. Using this method, it is possible to construct probes for the detection of virus diseases in plants and animals. The method can also be used to produce new plant strains which are resistant to a particular virus, or to provide immunity to animals to a specific virus.</p> |
