| 摘要 |
Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 81 (WEX1X2X3X4X5X6 X7X8X9X10X11X12X13X14X15X16X17X18D) in a beta subunit, and is characterized in that W is tryptophan, E is glutamic acid, D is aspartic acid, and X1-X6, and X8-X18 each independently indicate any amino-acid residue, wherein X7 is an amino acid selected from among alanine, valine, aspartic acid, threonine, phenylalanine, isoleucine and methionine; and X14 is glycine. |
