| 摘要 |
Provided are methods of evaluating binding or a compound to a biomolecule. The methods utilize 1D NMR of the compound and 1D or 2D NMR of the biomolecule-compound mixture, along with 2D HSQC or TROSY methodology if a biomolecule-compound complex is formed. These methods are useful for evaluating biomolecule binding with more than one compound, e.g., a library of compounds, either individually or in mixtures. The methods are also useful for NMR evaluation of the effects of competition between a compound and a known binder such as an inhibitor of biomolecule activity.
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