Method of making a hydrogel, hydrogel and formulation for carriers and/or substitute of connective tissues obtained using such method

摘要

A method of making a hydrogel for use as carrier and/or substitute of connective tissues includes providing an aqueous solution, adding and solubilizing a first electromagnetic radiation-polymerizable water-soluble polymer a polymerization regulator agent, for regulating the polymerization of the first polymer, and applying an electromagnetic radiation to achieve polymerization of the first polymer with a predetermined viscosity and obtain a sterile hydrogel. A second radiation-polymerizable water-soluble polymer, different from the first and is non-reactive to the regulator, is added before application of the electromagnetic radiation, the amounts of regulator agent, first polymer and second polymer being selected to control the final consistency and viscosity of the sterile hydrogel. The second polymer has a degree of polymerization that varies according to the intermolecular steric hindrance of the chains of the first polymer. A hydrogel and a formulation designed for use as carrier and/or substitute of connective tissues.

主权项

1. A method of making a hydrogel for use as a carrier and/or a substitute of connective tissue, comprising the steps of:
providing a predetermined amount of an aqueous solution; adding a predetermined amount of a polymerization regulator agent to said aqueous solution, for regulating the polymerization of a first electromagnetic radiation-polymerizable water-soluble polymer; adding, hydrating and/or solubilizing a predetermined amount of said first water-soluble polymer in said aqueous solution, to obtain a mixture; adding, hydrating and/or solubilizing a second electromagnetic radiation-polymerizable water-soluble polymer, different from the first water-soluble polymer, in said mixture; and applying an electromagnetic radiation of predetermined frequency to said mixture, for achieving the polymerization of said first polymer and said second polymer with a predetermined viscosity, and for obtaining a sterile hydrogel; wherein said second water-soluble polymer is non-reactive to said regulator agent and said second polymer has a degree of polymerization that varies according to intermolecular steric hindrance of chains of said first polymer, the amount of said regulator agent relative to said first polymer and the amount of said second polymer being selected to allow control of final consistency and viscoelasticity of said sterile hydrogel.