Method for the detection of polypeptide specific immune cells

摘要

The present invention relates to a method for polypeptide transfer into cells. The present invention further relates to the detection of polypeptide-specific immune cells and the priming, expansion and reactivation of polypeptide-specific T cells. Moreover the present invention relates to polypeptides of the methods of the present invention in combination with urea and their use for research, diagnosis or treatment and prevention of diseases in animals and humans.

主权项

1. A method for the detection of polypeptide-specific immune cells, comprising the following steps:
a) incubating polypeptides in a solution comprising cyanate ions and in the absence of urea, wherein the cyanate ions are present at a concentration in the range from about 1 to about 1000 mmol/l at temperatures in the range from −20° C. to 100° C. and at an incubation time of about several minutes to several days, b) incubating APC-containing cell cultures or body fluids with the polypeptides of step (a) in the presence of urea, c) incubating the APC-containing cell cultures or body fluids obtained according to step b) with immune cells or immune-cell-containing body fluids, and d) simultaneously and/or specifically detecting and/or quantifying various subtypes of immune cells which are specific against the polypeptides from step a).