发明名称 |
Process for purification of a fatty acid binding protein |
摘要 |
A process for purification of a fatty acid binding proteins such as, e.g., Sm14 of Pichia pastoris or type-3 FABP protein of Fasciola hepatica. The process includes the steps of: (a) performing lysis of cells containing the fatty acid binding protein to obtain a lysate; (b) clarifying the lysate obtained in step (a) to obtain a clarified lysate; (c) loading the clarified lysate in a column containing an anion exchange resin; (d) eluting proteins from the column by pH changes in the column; and (e) separating contaminant proteins from the fatty acid binding protein by gel-filtration. |
申请公布号 |
US9475838(B2) |
申请公布日期 |
2016.10.25 |
申请号 |
US201514886794 |
申请日期 |
2015.10.19 |
申请人 |
OURO FINO AGRONEGOCIO LTDA.;FUNDACAO OSWALDO CRUZ |
发明人 |
Tendler Miriam;Ramos Celso Raul Romero;Simpson Andrew J. G. |
分类号 |
C07K14/435;C07K1/36;C12N15/81;C07K14/395;A61K38/00;A61K39/00 |
主分类号 |
C07K14/435 |
代理机构 |
Caesar Rivise, PC |
代理人 |
Caesar Rivise, PC |
主权项 |
1. A process for purification of a recombinant Sm14 Schistosoma mansoni protein, said process comprising the steps of:
(a) performing lysis of cells containing the Sm14 protein to obtain a lysate; (b) clarifying the lysate obtained in step (a) to obtain a clarified lysate; (c) loading the clarified lysate in a column containing an anion exchange resin; (d) eluting proteins from the column by pH changes in the column; and (e) separating contaminant proteins from the Sm14 protein by gel-filtration, wherein said Sm14 protein is encoded by SEQ ID NO:3 and is expressed in Pichia pastoris. |
地址 |
Sao Paulo BR |