| 摘要 |
A human epidermal growth factor (EGF) is purifyed by (a) culturing recombinant yeast contg. human EGF gene, (b) concentrating the obtd. culture broth and centrifuging to obtain the supernatent, (c) diluting the supernatant with D.W. and adjusting the pH 7.0, (d) absorbing the obtd. soln. into anion exchange resin, (e) dissolving human EGF fraction with 10 mM tris buffer soln. contg. NaCl, (f) concentrating the obtd. fraction by using YC05 ultra filtrating membrane, and (g) filtering the concentrated soln. with 0.2 m filter membrane to obtain the final product. The method is useful for the prodn. of human EGF with a high purity.
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