Glycosaminoglycans

摘要

Heparan sulphate HS/BMP2 is disclosed, together with the use of HS/BMP2 in the repair and regeneration of bone tissue.

主权项

1. A composition comprising a solid or solidified scaffold coated or impregnated with a therapeutically effective amount of an isolated or substantially purified heparan sulphate composition comprising a heparan sulphate component, wherein the heparan sulphate component is at least 97% isolated or substantially purified heparan sulphate HS/BMP2, wherein following digestion with heparin lyases I, II and III and then subjecting the resulting disaccharide fragments to capillary electrophoresis analysis the heparan sulphate HS/BMP2 has a disaccharide composition comprising:Normalised weightDisaccharidepercentageΔHexUA,2SGlcNS,6S14.8 ± 3.0ΔHexUA,2S-GlcNS 4.9 ± 2.0ΔHexUA-GlcNS,6S11.1 ± 3.0ΔHexUA,2SGlcNAc,6S 4.8 ± 2.0ΔHexUA-GlcNS22.2 ± 3.0ΔHexUA,2S-GlcNAc 1.1 ± 0.5ΔHexUA-GlcNAc,6S10.1 ± 3.0ΔHexUA-GlcNAc31.1 ± 3.0.