| 摘要 |
PURPOSE:To obtain a lipid component having high gamma-linoleic acid content from cultured product by culturing a microbial strain capable of producing a lipid having high gamma-linoleic acid content, in a medium having high carbon source concentration. CONSTITUTION:The above medium having high carbon source concentration is the one containing about 3-10wt% organic carbon source preferably glucose, sodium scetate, etc. The N-source of the medium may be inorganic N-source such as nitrate as well as organic N-source such as yeast extract. The above lipid-producing strain belonging to Cunninghamella genus [e.g. Cunninghamella elegans (NRRL-1378, 1379, 1380, 1381)] is cultured in the above medium (usually a liquid medium). The cultivation is carried out at about 15-30 deg.C for about 4- 15 days. The microbial cells are collected from the cultured product, and the lipid is extracted from the cell by conventional method. A lipid component having high gamma-linoleic acid content can be obtained by this process. |
