| 摘要 |
<p>Purified human Le form interferon proteins are characterised and isolated by sodium dodecylsulphate polyacrylamide gradient electrophoresis as two major species of observed molecular weights 18 400 +/- 200 daltons and 20 100 +/- 200 daltons, and four minor species. Each indiridual pure interferon species has antiviral and anticellular activity, and each major species raises antibodies which neutralise the other. The pure interferon proteins are prepared from solutions thereof containing impurtities by antibody affinity chromatography optionally preceded by ligand affinity chromatography. Antibodies against the pure interferon proteins are useful in antibody affinity chromatography purification of crude interferon.</p> |
