EWOD DEVICE WITH CALIBRATED SERIAL DILUTION FUNCTION

摘要

In a method of performing dilution of a droplet in an EWOD device, a parent droplet is provided on an electrode array of the EWOD device, wherein the parent droplet has a first concentration of a species. A diluent droplet also is provided on the electrode array of the EWOD device. The method includes controlling actuation voltages applied to the electrode array of the EWOD device to join the parent droplet and the diluent droplet into a product droplet having a diluted second concentration of the species different from the first concentration in the parent droplet. The actuation voltages then are controlled to split the product droplet into one or more daughter droplets having the second concentration of the species. A dilution ratio may be calibrated based on the volumes of the droplets. Serial dilution steps may be performed to generate daughter droplets of different species concentrations at each step.

主权项

1. A method of performing dilution of a droplet in an electro-wetting on dielectric (EWOD) device comprising the steps of:
providing a parent droplet on a first portion of an electrode array of the EWOD device, wherein the parent droplet has a first concentration of a species; providing a diluent droplet on a second portion of the electrode array of the EWOD device; controlling actuation voltages applied to the electrode array of the EWOD device to join the parent droplet and the diluent droplet into a product droplet having a second concentration of the species different from the first concentration of the species in the parent droplet; calibrating a dilution ratio, wherein the dilution ratio is based on a ratio of the second concentration of the species in the product droplet to the first concentration of the species in the parent droplet, by measuring a volume of the parent droplet and a volume of the diluent droplet.