| 摘要 |
For the determination of a specific binding substance, a test solution containing at least three receptors R1, R2 and R3, of which R1 is capable of specific binding with R2 and R3 and with the substance to be determined, R2 conveys the bond to the solid phase and R3 carries a marker, is incubated, as receptor R1 use being made of a receptor which has at least two binding sites which bind specifically with an epitope of the substance to be determined, as R2 use being made of a conjugate of a partner P1 of a specifically binding pair and of a substance S, which corresponds to the substance to be determined or is a derivative thereof and has at least one epitope of the substance to be determined, the partner P1 either being bound to a solid phase or being able to be immobilised, and as R3 use being made of a complex which contains at least the substance S and a marker, the bound marker being separated from the unbound marker and the marker being measured in one of the two phases. |
