Hybrid Acquisition Method Incorporating Multiple Dissociation Techniques

摘要

A method is disclosed wherein parent or precursor ions are fragmented or reacted according to a first fragmentation or reaction mode, and when an ion of interest is detected the method then temporarily switches to a second mode of fragmentation or reaction. This enables a full un-biased MS/MS data set to be provided over a wide mass to charge ratio range with high-duty cycle, together with complementary detailed fragment data of interest, in a single experimental run or acquisition.

主权项

1. A method of mass spectrometry comprising:
mass filtering parent or precursor ions with a mass filter; transmitting the mass filtered parent or precursor ions into a fragmentation or reaction device operating in a first fragmentation or reaction mode; fragmenting or reacting the parent or precursor ions in the fragmentation or reaction device operating in the first fragmentation or reaction mode so as to produce first fragment or product ions; wherein the mass to charge ratio, or range of mass to charge ratios, of the parent or precursor ions transmitted by the mass filter is varied with time such that parent or precursor ions of different mass to charge ratios are fragmented or reacted by the first fragmentation or reaction mode at different times; monitoring for the detection of parent or precursor ions of interest and/or first fragment or product ions of interest, and wherein if parent or precursor ions of interest and/or first fragment or product ions of interest are detected then said method further comprises: halting the variation in the mass to charge ratios transmitted by the mass filter or otherwise operating the mass filter such that the mass to charge ratio, or range of mass to charge ratios, of the parent or precursor ions transmitted by the mass filter is static or remains constant for a first period of time; and then directing the parent or precursor ions transmitted by the mass filter during said first period of time into a fragmentation or reaction device operating in a second, different fragmentation or reaction mode of operation; and fragmenting or reacting the parent or precursor ions in the fragmentation or reaction device operating in the second fragmentation or reaction mode so as to produce second fragment or product ions.