REAGENT WELL HAVING A RETENTION FEATURE FOR RETAINING A LYOPHILIZED REAGENT

摘要

An article for performing a reaction includes a reagent well having a side wall, a bottom wall and an open upper end. A lyophilized reagent is retained within the well by a non-integral retention feature that is inserted into the open upper end of the well and fixed to a side wall above the reagent. An opening in the retention feature is smaller than the diameter of the reagent, but is sized to permit a pipette tip to be inserted into the well. The retention feature may be a collar fixed to the side wall and may include fingers extending axially into the well. The well and the retention feature may be correspondingly tapered. A method for reconstituting a lyophilized reagent includes the steps of drawing into a pipette tip attached to an automated pipettor an amount of a diluent and dispensing the diluent into the reagent well to thereby reconstitute the lyophilized reagent.

主权项

1. An article for performing a reaction comprising:
a reagent well including a side wall, a bottom wall, and an open upper end defined by a top surface; a lyophilized reagent disposed within the reagent well; and a retention feature that is not integral with the reagent well, wherein the retention feature is inserted into the open upper end of the well and fixed to the side wall above the lyophilized reagent, wherein the retention feature defines an opening that is smaller than the diameter of the lyophilized reagent, such that the retention feature retains the lyophilized reagent within the reagent well, and wherein the opening is sized to permit an end of a pipette tip to be inserted into the well.