发明名称 |
NUCLEIC ACID CONSTRUCTS ENCODING REPROGRAMMING FACTORS LINKED BY SELF-CLEAVING PEPTIDES |
摘要 |
The disclosure relates to a method of reprogramming one or more somatic cells, e.g., partially differentiated or fully/terminally differentiated somatic cells, to a less differentiated state, e.g., a pluripotent or multipotent state. In further embodiments the invention also relates to reprogrammed somatic cells produced by methods of the invention, to chimeric animals comprising reprogrammed somatic cells of the invention, to uses of said cells, and to methods for identifying agents useful for reprogramming somatic cells. |
申请公布号 |
US2017067081(A1) |
申请公布日期 |
2017.03.09 |
申请号 |
US201615354604 |
申请日期 |
2016.11.17 |
申请人 |
Whitehead Institute for Biomedical Research |
发明人 |
Jaenisch Rudolf;Carey Bryce Woodbury |
分类号 |
C12N15/86;C07K14/47 |
主分类号 |
C12N15/86 |
代理机构 |
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代理人 |
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主权项 |
1. A nucleic acid construct comprising at least three coding regions, a first coding region encoding a first reprogramming factor, a second coding region encoding a second reprogramming factor, and a third coding region that encodes a third reprogramming factor, wherein the first and second coding regions are linked by a nucleic acid that encodes a first self-cleaving peptide and the second and third coding regions are linked by a nucleic acid that encodes a second self-cleaving peptide so as to form a single open reading frame, wherein expression of the first, second, and third reprogramming factors is sufficient for reprogramming a mammalian somatic cell to pluripotency, and
wherein the nucleic acid construct does not comprise Oct 3/4, Sox 2 and Klf4 ligated across the 2A sequence of foot-and-mouth disease virus in the order of Oct 3/4, Klf4 and Sox2. |
地址 |
Cambridge MA US |