KETOREDUCTASE POLYPEPTIDES FOR THE PRODUCTION OF AZETIDINONE

摘要

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

主权项

1. A polynucleotide sequence encoding an engineered ketoreductase polypeptide having ketoreductase activity, wherein said ketoreductase polypeptide is at least about 85% identical to SEQ ID NO:4, and wherein the ketoreductase polypeptide comprises histidine at position 40.