METHOD FOR NEUTRALIZING HEPATITIS C VIRUS, FULLY HUMAN MONOCLONAL ANTIBODY AGAINST HEPATITIS C VIRUS (VARIANTS), COMPOSITION OF FULLY HUMAN MONOCLONAL ANTIBODIES AGAINST HEPATITIS C VIRUS AND HYBRID MOUSE/HUMAN PRODUCER CELL LINE OF FULLY HUMAN MONOCLONAL ANTIBODIES AGAINST HEPATITIS C VIRUS (VARIANTS)

摘要

The invention relates to the field of biotechnology, and specifically to methods and techniques for neutralizing the hepatitis C virus, and specifically to antibodies against the hepatitis C virus, and can be used in medicine, the pharmaceutical industry and related areas of science and technology. Proposed is the use of fully human monoclonal antibodies—RYB1, RYB2 and RYB3—and of a composition based thereon for the prevention and treatment of hepatitis C. Said antibodies are produced by cultivation using hybrid BIONA-RYB1, BIONA-RYB2 and BIONA-RYB3. The effectiveness of the antibodies is due to said antibodies binding epitopes, namely Ep1, Ep2 and Ep3 of E2 protein of the hepatitis C viral envelope, respectively. The present invention has demonstrated a neutralizing activity of the antibodies in a model system of infection of human cells in a culture. It has been shown that use of the claimed group of inventions provides for more reliable antibody binding of the hepatitis C virus.

主权项

1. A method for neutralizing the hepatitis C virus by contact with antibodies which bind to epitopes of E2 protein of the hepatitis C viral envelope, which is characterized in that contact is made by introducing into the system at least one antibody capable of reacting with at least one epitope selected from the group of epitopes Ep1, Ep2, Ep3, in which epitope Ep1 of the E2 protein of hepatitis C virus isolate H77 is a conformation of a continuous amino acid sequence contained in the region HPEATYSRCG (SEQ ID NO: 30) and containing the amino acids Ser and Arg of this region, epitope Ep2 of the E2 protein of hepatitis C virus isolate H77 is a conformation of a continuous amino acid sequence contained in the region VCGPVYCF (SEQ ID NO: 32) and containing the amino acid Tyr of this region, and epitope Ep3 of the E2 protein of hepatitis C virus isolate H77 is a conformation of a continuous amino acid sequence contained in the region HPEATYSRCGSGPWITP (SEQ ID NO: 34) and containing both Ser amino acids and the amino acid Arg of this region.