Microfluidic Device For Real-Time Clinical Monitoring And Quantitative Assessment Of Whole Blood Coagulation

摘要

A microfluidic coagulation assessment device includes a plurality of microchannels, with a blood sample driven through the microchannels at a substantially constant flow rate. A controller is configured to, in combination with a timer and a pressure sensing device, determine a first pressure value (or flow value) at an initiation of flow, a first time (Tpg) at which a second pressure value is about twice the determined first pressure value, and a second time (Tpf) at which a third pressure value is about (1+e) times the determined first pressure value and establish a subject coagulation model predictive of channel occlusion therefrom.

主权项

1. A microfluidic coagulation device, comprising:
at least one substrate defining a plurality of microchannels; a first port at a first end portion of the substrate, the first port connecting to first ends of the plurality of microchannels; a second port at a second end portion of the substrate, the second port connecting to second ends of the plurality of microchannels; a first sensing device configured to determine a pressure value in, or relating to, a pressure across the plurality of microchannels; a timer; and a controller configured to determine, in combination with the first sensing device and the timer, a first pressure value at an initiation of flow, a first time at which a second pressure value is determined to be about twice the determined first pressure value, and a second time at which a third pressure value is about (1+e) times the determined first pressure value, and further configured to establish a subject coagulation model predictive of channel occlusion in accord with the relationΔP(t)ΔP(0)=1+t-TpgTpf-Tpg wherein Tpf is the second time and Tpg is the first time, and wherein a differential pressure or flow rate/shear applied across the first port drives a blood sample across the plurality of microchannels at a substantially constant flow rate.