Preparation and/or formulation of proteins cross-linked with polysaccharides

摘要

Therapeutic compositions and/or formulations are provided, comprising: at least one cross-linked protein matrix, wherein the at least one cross-linked protein matrix comprises at least one protein residue and at least one saccharide-containing residue, and methods of producing the same. The cross-linked protein matrix may be derived from cross-linking a full length or substantially full length protein, such as tropoelastin, elastin, albumin, collagen, collagen monomers, immunoglobulins, insulin, and/or derivatives or combinations thereof, with a saccharide containing cross-linking agent, such as a polysaccharide cross-linking agent derived from, for example, hyaluronic acid or a cellulose derivative. The therapeutic compositions may be administered topically or by injection. The present disclosure also provides methods, systems, and/or kits for the preparation and/or formulation of the compositions disclosed herein.

主权项

1. A composition, comprising at least one cross-linked protein matrix, wherein the at least one cross-linked protein matrix comprises:
i) between 1-250 mg/mL of at least one protein residue derived from at least one amine-bearing protein; ii) at least one saccharide-containing cross-linking residue derived from at least one saccharide-containing cross-linking molecule comprising one or more carboxyl groups; and iii) at least one intermolecular cross-linkage comprising an amide bond between an amine of the at least one protein residue and a carboxyl group of the at least one saccharide-containing cross-linking molecule.