UNIVERSAL DNA PROFILING

摘要

A method for characterizing at least a portion of the biodiversity of a sample. The method includes the steps of: (i) obtaining a sample having nucleic acid from a plurality of different organisms; (ii) extracting at least a portion of the nucleic acid from the sample; (iii) optionally performing a whole-genome amplification of the extracted nucleic acid; (iv) optionally performing a second, targeted amplification; (v) sequencing the amplified nucleic acid to obtain sequence data comprising a nucleic acid sequence for at least some of the plurality of different organisms; (vi) querying, using the obtained sequence data, a sequence database, where querying the sequence database identifies one or more of the plurality of different organisms; and (vii) determining, using the identified one or more of the plurality of different organisms, a characteristic of the sample.

主权项

1. A method for characterizing at least a portion of the biodiversity of a sample, the method comprising the steps of:
obtaining a sample, the sample comprising nucleic acid from a plurality of different organisms; extracting at least a portion of the nucleic acid from the sample; filtering the sample to enrich the sample for target organisms; performing an amplification of the extracted nucleic acid; sequencing the amplified nucleic acid to obtain sequence data comprising a nucleic acid sequence for at least some of the plurality of different organisms; querying, using the obtained sequence data, a sequence database, wherein querying the sequence database identifies one or more of the plurality of different organisms in the sample; and determining, using the identified one or more of the plurality of different organisms, a characteristic of the sample.