Method of differentiating mammalian progenitor cells into insulin producing pancreatic islet cells

摘要

The invention relates to methods for differentiating progenitor cells into insulin producing pancreatic islet cells and compositions and methods for using such cells.

主权项

1. A method for differentiating progenitor cells into insulin-producing pancreatic cells, comprising:
(a) incubating the progenitor cells in serum-free pre-induction media comprising a histone deacetylase inhibitor (HDACI); and, subsequently, (b) incubating the progenitor cell in induction media comprising knock-out replacement serum; for a time sufficient to differentiate the progenitor cells into insulin producing pancreatic cells wherein the progenitor cells are selected from the group consisting of hepatic stem cells, hepatoblasts, or hepatic committed progenitors.