Markers of definitive endoderm

摘要

Disclosed herein are reagent-cell complexes comprising one or more definitive endoderm cells. Also described herein are compositions for detecting definitive endoderm. Method of enriching, isolating and/or purifying definitive endoderm cells are also disclosed.

主权项

1. An in vitro method of producing cells which express thyroid transcription factor 1 (TITF1) from mammalian definitive endoderm cells comprising the steps of:
(i) culturing mammalian definitive endoderm cells with an effective amount of Wnt3a for at least 7 days, (ii) enriching for cells which express TITF1.