MALARIA ANTIGEN SCREENING METHOD

摘要

The invention provides a method of identifying an antigen from a pathogen or a disease antigen comprising the use of an adenoviral vector array comprising two or more different adenoviral vectors, wherein each adenoviral vector comprises a nucleic acid sequence encoding a different antigen of a pathogen. The adenoviral vectors are administered to antigen presenting cells (APCs) in vitro or to an animal in vivo. The immunogenicity of the antigen is measured by screening for an immune response from effector T lymphocytes in vitro and by screening for the absence of pathogen-induced disease onset in vivo.

主权项

1. A method of identifying an antigen from a pathogen, which method comprises
(a) preparing an adenoviral vector array comprising two or more different adenoviral vectors, wherein each adenoviral vector comprises a nucleic acid sequence encoding a different antigen of a pathogen, (b) contacting antigen presenting cells (APCs) with the adenoviral vector array, wherein each different adenoviral vector transduces an APC such that the nucleic acid sequences of the different adenoviral vectors are expressed and the different antigens are produced in the APCs, (c) incubating the APCs with effector T lymphocytes obtained from a mammal immunized with the pathogen, and (d) screening for an immune response from the effector T lymphocytes, wherein an immune response from an effector T lymphocyte contacting an APC indicates T lymphocyte recognition of the antigen produced by the APC, whereupon the antigen is identified.